Helicases use the energy derived from nucleoside Synonyms, uvr502; srjC; uvrE; dar-2; dda; mutU; pdeB; rad; recL. Accession IDs, EG11064 (EcoCyc) b3813. ECK3808 P03018 (UniProt), Length, 2163 bp / 720 Oct 15, 2013 UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in Oct 19, 2018 UvrD protein can self-associate into dimers and tetramers , and its assembly state regulates its properties. A UvrD monomer can processively UvrD/REP helicase N-terminal domain Provide feedback. The Rep family helicases are composed of four structural domains. The Rep family function as dimers.
Unreviewed - Annotation score: Annotation score:2 out of 5. The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score cannot be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein. The kinetic mechanism by which the DNA repair helicase UvrD of Escherichia coli unwinds duplex DNA was examined with the use of a series of oligodeoxynucleotides with duplex regions ranging from 10 to 40 base pairs. Single-turnover unwinding experiments showed distinct lag phases that increased with duplex length because partially unwound DNA intermediate states are highly populated during However, UvrD unwinds duplex DNA with a specific polarity (39, 53, 62, 67). Therefore, in order for UvrD to unwind toward the mismatch it must be loaded onto the appropriate strand to unwind with its known polarity.
The new, updated "Virtual Rosary - The Luminous Mysteries" video can be found here - h Abstract.
The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists. En slitstark, välsittande och smidig paddel- och jolleväst.
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UvrD is a superfamily I DNA helicase with well documented roles in excision repair and methyl-directed mismatch repair (MMR) in addition to poorly understood
UvrD helicase is required for nucleotide excision repair, although its role in this process is not well defined. Here we show that Escherichia coli UvrD binds RNA
Jun 9, 2010 Atomic resolution structures of UvrD-like helicases complexed with DNA in the presence of AMPPNP, ADP·Pi, and Pi reveal several salient
Nov 19, 2013 UvrD Oligomeric State 48. UvrD Oligomeric State 01:05:11.
that UvrD can pull RNA polymerase backward suggests that the role of UvrD in both NER and collision avoidance is more complex than previously thought. For helicase aficionados, the subtle aspects of UvrD mechanism are intriguing. UvrD is known to load at single-stranded/ double-stranded junctions and, depending on its oligmeric state UvrD, a highly conserved helicase involved in mismatch repair, nucleotide excision repair (NER), and recombinational repair, plays a critical role in maintaining genomic stability and facilitating DNA lesion repair in many prokaryotic species. UvrD helicase plays essential roles in multiple DNA metabolic processes, including methyl-directed mismatch repair. UvrD monomers can translocate along single-stranded DNA, but self-assembly or interaction with an accessory factor is required to activate processive DNA unwinding in vitro.
Once the UvrD monomer reached the Cy3-labeled and fluorescein-labeled 5’-ends of the ssDNA, the fluorescence intensity was enhanced and quenched, respectively. 6). UvrD also participates in the UvrABC nucleotide excision repair pathway by removing the 12–13-base oligonucleotide containing a pyrimidine dimer or bulky adduct (3). Additional functions for UvrD have been proposed, consistent with the pleiotropic nature of uvrD mutants (4, 5, 7), including roles in replication and recombination (8–12). Product Class: Other Tte UvrD Helicase Need assistance designing LAMP primers? Use the NEB LAMP Primer Design Tool.
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ECK3808 P03018 (UniProt), Length, 2163 bp / 720 Oct 15, 2013 UvrD is a DNA helicase involved in several DNA repair pathways. We report here crystal structures of Deinococcus radiodurans UvrD (drUvrD) in Oct 19, 2018 UvrD protein can self-associate into dimers and tetramers , and its assembly state regulates its properties. A UvrD monomer can processively UvrD/REP helicase N-terminal domain Provide feedback. The Rep family helicases are composed of four structural domains. The Rep family function as dimers.
DNA helicases are enzymes capable of unwinding double-stranded DNA (dsDNA) to provide the single-stranded DNA template required in many biological processes. Among these, UvrD, an essential DNA repair enzyme, has been shown to unwind dsDNA while moving 3′-5′ on one strand. *Attention* - We have re-recorded the audio and re-created this video. The new, updated "Virtual Rosary - The Luminous Mysteries" video can be found here - h
An oligomeric form of E. coli UvrD is required for optimal helicase activity. Pre-steady-state chemical quenched-flow techniques were used to study DNA unwinding catalyzed by Escherichia coli UvrD helicase (helicase II), a member of the SF1 helicase superfamily. DNA helicase II (sometimes called UvrD) then comes in and removes the excised segment by removing the base pairing.